The 18S rRNA primers were previously shown to recognize Drosophila and B. anynana 18S rRNA genes, and 18S appears to have a constant expression level across multiple distinct samples, serving as an adequate endogenous control [ 27].
These concordant differences at multiple, independent, gene loci led Won et al.[ 29] to suggest that the southern lineage might warrant consideration as a distinct species, but additional samples and analyses of independent nuclear gene loci were needed to assess this hypothesis.
A non-spiked sample was retained from the same pool of serum for each round and analysed in parallel; we subtracted the values obtained for this non-spiked sample from each laboratory's measured spiked sample, which allowed us to determine the accuracy of the method as a distinct issue from contamination during sample collection.
The AFM height data was represented as a distinct height value of the sample in a finite number of pixels (512 × 512 point scan) [ 29].
First, we treated our Sierra Nevada sample as a distinct group, because we were interested in resolving the phylogenetic relationships of this disjunct population using data from both mtDNA and nuclear genes.
For the 371 participants with two home visits, we selected homes for our sample according to the following criteria: If a participant moved between her baseline and 6-month home visit, each of her homes is present in our housing sample as a distinct home.
We used the two populations of B. azoricus as distinct samples (never pooled) in order to test whether a "site effect" occurred between Menez Gwen (850 m) and Rainbow (2500 m) and to get partial replicates when comparing B. azoricus and B. puteoserpentis.
Two sites each were sampled at Subri reef (Persian/Arabian Gulf) and Fahal Island (Sea of Oman), which were previously grouped together, but treated as distinct sampling locations in this study.
Some of these products were shipped in divided shipments and treated as distinct samples.
The Indian H. pylori sequences were clustered within the European portion of the network, wherein the first domain identifies a separate branch, encompassing the majority of the Ladakhi samples, as a distinct sub-population of hpAsia2 within the European variability, and remarking the isolation of the human host population.
The T.alp-S sample appears as a distinct cluster, with one exception.
