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Major points: 1) Ssas as a direct tRNA importer.
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The upregulation of tRNAs may have resulted as a direct consequence of B. cenocepacia growth in sputum-enriched BSM which effectively made it a nutrient-rich medium.
According to the context, these scores are used here according to the interpretation that they reflect aminoacylation potential, or as a direct measure of alignment quality with the 'standard' bacterial tRNAs used as reference sequences by TFAM.
Specifically, Ψ-1956 (1915) contacts the D stem of tRNA in the A site (positions 11 and 12) and Ψ-1958 Ψ-1958 1917mmedistely adjacent to brimmediatelyntadjacent well as directocontacts to A-site tRNA; they are important for the conformational flexibridge of helix 69 and their loss affects suB2ait assocontacts dissociasion, and translation termination [ 17, 58- 61].
Two different programs (tRNAscan-SE and ARAGORN) did not detect a tRNA for tryptophan (W); instead, this tRNA was identified as a tRNA for selenocystein.
Mass spectrometric profiling has revealed S-geranylation as a new tRNA modification and identified SelU as the tailoring enzyme in bacterial cells.
As mentioned above, we observed a direct interaction between Ssa proteins and tRNAs, which is governed by a mode different from that for recognition of protein substrates by Hsp70.
Vectors with six tandemly arranged tRNA genes, as well as with a single tRNA gene and the corresponding 5' and 3' flanking sequences, were generated, based on previous findings [6].
Thus, we were able to show a direct link between the vital tRNA binding property of EF-Tu and polymerase activity.
The results of these studies demonstrate that intracellular levels of primer tRNA can have a direct effect on HIV-1 infectivity and further support the role for PBS-tRNA complementarity in the primer selection process.
We note that GI2 is located directly downstream of tRNA-Phe and contains a direct repeat (45 bp) of the 3' end sequence of tRNA-Phe.
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