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Briefly, after cells were treated with RAC1 inhibitor (100 μM NSC23766) for 24 and 48 h, all cells were collected and diluted with phosphate-buffered saline (PBS) containing 1% BSA as a dilution buffer to a concentration of 5 × 10 cells/mL.
One long-time burner told me he's disillusioned with what he sees as a dilution of Burning Man culture on the playa, and no longer wants to attend.
Mr. Dobbs -- who, like Mr. Coffey, was in charge of business programming for the entire CNN family of networks -- saw that as a dilution of the CNNfn brand.
Employers don't like it because it calls for more imaginative arrangements and the trade unions long discouraged it as a dilution of labour.
"The concept of trading with other people is very powerful for some, who may see corporate partnerships as a dilution of the brand," she says.
The decision to place new "supporters" into the trade union section of the electoral college has been spun as a dilution of the unions power, when in fact it is a dilution of the power of the supporters themselves.
Similar(24)
Samples were considered seropositive if they were confirmed as positive at a dilution of >1 40.
Antibody against β-actin was used as a control at a dilution of 1∶7500.
Anti-mouse IgG-alkaline phosphatase conjugates were used as secondary antibodies at a dilution of 1 3000.
β-actin was used as loading control at a dilution of 1 20000.
Mouse anti-HA (Roche) was used as the primary antibody at a dilution of 1 : 1000.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com