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Iron (III) staining was performed on free-floating sections as a described improved method for Perls' stain [49].
FISH detection using the TSA amplification system was followed as a described in Lyles et al. (2006).
The peak-value of the stimuli-induced [Ca2+]cyt transient was calculated as a described by Cobbold and Rink [ 5] with some modifications [ 6].
On day 2, we removed the SN cell body and 18 hr later, we photoconverted dendra2 from green to red as a described in Wang et al. (2009), with slight modifications, using an X-cite series120Q excitation light (a 120-watt lamp), delivering two 5 s pulses of UV illumination with a 10 s interval.
Similar(56)
a as described in 'Material and methods'.
SmUBQ10 was used as a reference as described previously [ 12].
and used as a negative control as described previously [ 29].
As a novelist, I described it fictionally.
A read described as chillier than Gone Girl.
Column identities are as described in (a).
Cells were sporulated as described in (A ).
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