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We first documented ATM/ATR activation by monitoring the pS/TQ immunostaining signals, as a dependable indicator of ATM/ATR activation in the Drosophila midgut.
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We determined whether the pS/TQ signal is associated with DNA damage and is a dependable indicator of ATM/ATR activity in the adult Drosophila midgut by means of ionizing radiation (IR) as a DNA damage inducer and by means of flies with an ISC/EB-specific knockdown of ATM, ATR, or both under the esg ts>GFP genotype.
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