Embryonic stages were determined according to the copulation plug date (E0.5 was counted as the first day a copulation plug was observed).
At his news conference Mr. Refn jokingly described his collaboration with Mr. Gosling as a mental copulation (he used a more explicit term), and the pair walked the red carpet arm in arm.
Experimental litters were generated by overnight mating, with the day of finding a copulation plug designated as E0.5.
Embryos were generated by overnight matings, with the day of finding a copulation plug designated as embryonic day (E) 0.5.
Throughout this work, the first day after finding a copulation plug was counted as embryonic day 0.5 (E0.5).
eNOS−/− were obtained from Jackson Laboratories (strain B6.129P2-Nos3tm1Unc/J). Homozygous eNOS−/− mice were mated, and the presence of a copulation plug was denoted as day 0.5 of pregnancy.
In rare cases, old ergatoid males displayed alleviated biting of pigmented ergatoid male pupae shortly before adult eclosion, as well as copulation attempts to dark pupae of female sexuals and winged males.
Males in many species discharge urine on females as a preliminary to copulation.
However, prednisolone treatment causes intestinal injury, what could hamper the parasite attachment to the intestinal wall, and as a result affects copulation and, consequently, decreases the number of eggs eliminated in the feces.
The males bite at the sides of the females as a prelude to copulation.
