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This inhibitory effect was recognized as a bactericidal effect.
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However, at higher concentrations (2 %), as used in this work, it has a bactericidal effect due to precipitation and/or coagulation of the cytoplasm, probably caused by protein crosslinking [20], and it presented an excellent diffusion into dentinal tubules.
In our study the action of especially the Manuka honey against P. gingivalis biofilms seems to be not only due to a bactericidal effect of methylglyoxal as is was itself non effective under those conditions.
A bactericidal effect is defined as a 3 Log decrease in the CFU/ml or a 99.9%% kill over a specified time [ 25].
For the definition of multidrug resistance we considered antimicrobial agents which have a bactericidal effect on P. aeruginosa and can be administered as effective monotherapy.
This antimicrobial activity is probably related to the mechanism of action of the protein, which exerts a bactericidal effect by producing hydrogen peroxide and can be used as a natural repellent.
This setback served as an impetus for rationally designing a library of next-generation antibiotics that exhibit a bactericidal effect on resistant P. acnes and exert an immunomodulatory function to reduce inflammation.
However at higher concentrations the pigment had a bactericidal effect.
As the swarming capacity reduces, the availability of nutrients also reduces and hence there has been a bactericidal effect.
Lysozyme has a bactericidal effect by destroying cellular walls of bacteria.
Therefore, LEO showed a bactericidal effect against the SRB D. alaskensis.
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