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The poll worker who watched as I inserted my ballot to be scanned could easily view my votes, as could anyone with a wandering eye standing near the scanning machine.
Typing cold, flat figures became the highlight of my day, and I felt better and better as I inserted numbers into that worksheet.
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There is a lot of wisdom in these speeches, so I hope that you will indulge me as I insert excerpts from previous Presidential addresses into my own remarks today.
"My trick when we got in was that I inserted as much ambitious and bombastic language as possible on open data into the speeches I wrote for George and David.
cDNAs were cloned as BamHI/NotI inserts into the multiple cloning site of pcDNA5 FRT/TO that adds a C-terminal GFP tag to the expressed protein.
I refreshed my email even as I deftly inserted the change, but the turnstile didn't budge.
PAK4 cDNA was cloned into this plasmid as a BamHI/NotI insert at the multiple cloning site just upstream of, and in frame with, the GFP coding sequence.
KEAP1 (NM_203500.1) was amplified from EST IMAGE 3163902 and subcloned as a BamHI/NotI insert into a modified pFastbacDualbaculoviral vector containing an N-terminal DAC tag [ 26] with a TEV (tobacco etch virus) protease site downstream of the DAC tag.
To support all functions of SBML Events, we implemented an event handler as follows: (i) Insert all Events that are related to a given Trigger to a queue.
The wild-type ffluc was cloned into pMV306hsp as a HindIII-SalI insert.
The amplified fragment was ligated into SmaI-digested pBluescript II KS+ and then into the plant transformation vector, pCAMBIA1300, as a BamHI-PstI insert.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com