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First, in the present study the cases were specially selected for evaluation of data quality and contained many artificially incorporated pitfalls in data extraction.
Amplification was performed using biotinylated primers to the flanking sequences allowing depletion of the artificially incorporated cDNA ends (Additonal file 1, figure S1).
The development of the pscA gene duplication method presented here, thus, enables not only a quick and large-scale preparation of the RC complex from C. tepidum but also site-directed mutagenesis experiments on the artificially incorporated 6xHis-tag-pscA gene itself, since the expression of the authentic PscA/PscA homodimeric RC complex could complement any defect in mutated His-tagged PscA.
Alternatively, such a pre-miRNA insert can also be artificially incorporated into the intron region of a cellular gene in the skin.
From a prediction perspective, the actual ratio of normal to malignant cells that evolves in treatment-driven ESS depends on the fitness numbers artificially incorporated into the game for any particular strategy.
Similar(51)
Following a short enrichment period, artificially contaminated food samples were subjected to a triplex PCR assay, which incorporated published primers for each food pathogen, a protocol for sample collection, and a PCR procedure designed specifically for the assay.
Artificially Colored.
Mix until barely incorporated.
"We incorporated," he said.
Stir until well incorporated.
Beat just until incorporated.
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