Exact(2)
Immediately thereafter, the animals were killed, the subclavian and carotid arteries rapidly removed en bloc, the vessel system flushed with 0.9% saline, and the arteries fixed by means of perfusion with 4% buffered formalin under physiologic pressure and overnight immersion.
Koskinas et al., who studied the flow in porcine coronary arteries, fixed the WSS threshold at 1.2 Pa, a value previously used in a study of flow in human coronaries; In a second study of porcine coronary arteries by the same group, a threshold of 1 Pa was chosen; Samady et al., in a study of human coronary arteries, defined low TAWSS as <1 Pa and high TAWSS as ≥2.5 Pa.
Similar(58)
After 20 minutes of incubation, these arteries were fixed in 4% formaldehyde for 30 minutes.
After imaging, arteries were fixed in 10% buffered formalin for ≥24 hours.
Immunohistochemistry For immunohistochemistry, arteries were fixed with phosphate-buffered (pH 7.4) formaldehyde (4%, vol./vol)./vol
After the collection, the mesenteric arteries were fixed by the same protocol.
Samples of native, defrozen, and decellularized arteries were fixed with 2.5% glutaraldehyde for 1 h and stored in sodium cacodylate 0.1 M.
Serial sections from the stented arteries were fixed in phosphate buffered saline (PBS) containing 10% formalin, dehydrated by treatment with solutions of increasing alcohol content (70%, 85 %, 95, and 100%), followed by xylene and then embedded in paraffin.
After the collection of carotid arteries from the above, the carotid artery was fixed in 4% paraformaldehyde for 12 hours, embedded in paraffin and sectioned for immunohistostaining.
With the volume of the artery then fixed, the pressure difference across the arterial wall (transmural pressure) is zero and intraarterial equals extraarterial pressure.
The right hepatic artery was fixed to the tumor, and we performed a complicated resection.
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