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High K+ induces biphasic contractions in rabbit arteries [ 15], rat basilar arterial rings [ 16] and mouse aorta [ 17], whereby the tonic rise in force is actually accompanied by a decline of intracellular Ca2+.
Briefly, the arterial rings were isolated from the main artery and first and second branches of the mesenteric artery, and they were quickly frozen in liquid nitrogen and kept in 10% trichloroacetic acid (TCA -acetone aTCA -acetonenight.
VOSO4 produced dose-dependent constriction of pulmonary arteries in isolated perfused lungs and pulmonary arterial rings and a right shift of the acetylcholine-dependent vasorelaxation curve.
Thus, the present study was designed to further investigate the direct effects of mepivacaine on these vessels in vitro using swine lingual and pulmonary arterial rings and compared with the coronary artery.
Intratracheal instillation of APP increases pulmonary artery resistance in isolated buffer-perfused lungs, and APP constricts isolated arterial rings.
However, despite the small sample size and insufficient power analysis, we believe that the data provide accurate and reliable information on the effect of mepivacaine on arterial rings, and the findings are useful for further investigation of craniofacial arteries.
The arterial rings that were isolated from the first branch of the rabbit mesenteric artery were cultured with an FBS-supplemented DMEM in the absence and presence of 10 ?M Y-27632.
Preincubation of these arterial rings with EC50's of PDTC or Vit.
Arterial rings were suspended in organ baths and changes in isometric tension were measured.
Rat mesenteric arterial rings were isolated for isometric force recording and electrophysiological studies.
Arterial rings were suspended in organ baths and tension was recorded isometrically.
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