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Processing was observed and data were collected during each of the following notable events: specimen arrival, specimen accessioning, control preparation, specimen preparation, instrument preparation, worklist preparation, instrument processing, and result verification.
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All samples were wild caught, 'dead on arrival' specimens provided by a Canadian importer of marine ornamental fishes.
Upon arrival specimens were stored at -70°C until testing.
After laboratory arrival, the specimen was cultured on cysteine lactose electrolyte deficient agar (CLED) by using a calibrated loop that can deliver 0.002 ml of urine.
Upon arrival, the specimens were resuspended in the medium by vortexing, and a portion of 1 ml centrifuged at 15,000 × g for 15 min. The supernatant was removed and the sediment washed in 500 μl phosphate-buffered saline, pH 7.3.
Difficulties in obtaining a clean urine sample, transportation limitations that can prevent timely arrival of specimens from the clinic to a microbiological facility, and use of shop-bought antibiotics or yaa-chud, which has been reported to contain antibiotics, 13 could inhibit culture growth.
A morphologic diagnosis may be obtained within 10 min of specimen arrival in the electron microscopic facility.
First, the time to results is approximately one hour from arrival of the specimen at the laboratory.
Because the actual time of specimen arrival is not searchable on our system, the representativeness of this electronic data as a proxy for total test turnaround time was verified by a manual audit of 200 Medipath files.
The laboratories will be asked to complete the following tasks for DUTY study samples: 1. Log the date and time of specimen arrival on the secure DUTY web-based database.
An analysis of lag times in Australian cultivated plants using planting lists to indicate date of arrival and herbarium specimens as evidence of naturalization estimated a mode time of 149 years between introduction and naturalization [6].
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