DNA-damage checkpoints are activated to arrest cells and promote survival upon genotoxic challenge.
Compound 9e was also shown to arrest cells in the G2/M phase of the cell cycle and inhibit the polymerization of tubulin.
Similar to vinca alkaloids, maytansine and its analogs emtansine (DM1) which are microtubule-targeted drugs binding to tubulin at the vinca binding site can depolymerize microtubules and arrest cells in mitosis, resulting in a powerful anti-mitotic activity [7 10].
About the mechanism of enhanced radiosensitivity, Turner et al. reported metallic materials may arrest cells at the G2/M phase, the most radiosensitive phase of the cell cycle [33].
Nocodazole treatment is widely used to arrest cells in mitosis.
Furthermore, HSV-1 infection is known to arrest cells in G1 [45].
We used colchicine or nocodazole to arrest cells in G2/M phase (Fig. 5A).
The compounds which arrest cells in M phase are mostly tubulin-targeting agents.
After 24 hours the serum was removed for 48 hours to arrest cells in G0/G1.
