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All 128 arrays were treated in 2 tissues, root and shoot 50-50.
Before antibody staining the arrays were treated with ReBlot Plus Mild Antibody Stripping Solution (Chemicon, Temecula, CA) 1X for 15minutes at room temperature, rinsed 2 times for 5minutes in PBS 1X, and then blocked for 1 hour at room temperature in blocking solution (2gr I-Block - Tropix, Bedford, MA - and 0.1% Tween-20 in 1l of PBS 1X).
The long-oligonucleotide arrays were treated the same except that the last wash step was omitted.
Finally, arrays were treated with the Stabilization and Drying Solution (Agilent Technologies) for 30 seconds at room temperature.
In the subsequent data preprocessing, raw intensity data from the spotted arrays and Agilent arrays were treated equally.
Next, the same arrays were treated in concentrated ammonia at r.t. for 2 h and then scanned.
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When the TiO2 nanotube arrays are treated in the niobium isopropoxide solution, the conversion efficiency is further raised to 9.1% and only suffers a 6.6% relative decrease even under a 180° bending.
In this model, SiNW arrays are treated as thermal resistors in parallel with the parylene matrix.
In the case control assay pattern, the control arrays are treated as the reference pool.
Duplicated probes on the array were treated independently during normalization and statistical analyses.
All the 8704 features on the array were treated as individual genes although each cDNA and control spots were duplicated on the array.
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