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The combined set of arrays was subsequently quantile normalized.
Variation in expression between arrays was subsequently clustered by wing, forewings were further separated by morph, and sections of forewings from the same individual tended to cluster within morph.
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The arrays were subsequently washed and stained.
Arrays were subsequently washed, stained and scanned using an Affymetrix GeneChip®-related software.
The arrays were subsequently washed, stained and scanned as in [22].
Arrays were subsequently stained with Streptavidin-Cy3 and scanned with a high resolution Illumina scanner to determine fluorescence intensities.
The probe sets for the remaining arrays were subsequently mapped to Ensembl transcripts using the mappings provided by BioMart.
The 3D transfected cell arrays were subsequently employed for large scale reporting on the dynamic changes in TF activity in response to E2 treatment.
Hybridisations were done overnight for ~19 h at 60°C and arrays were subsequently washed.
The arrays were subsequently washed, stained and scanned according the manufacturer's instructions (GeneChip® Expression Analysis Technical Manual; Affymetrix).
The cDNA arrays were subsequently hybridized with radioactive labeled cDNA probes from the different passages of the RA patients.
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