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Taguchi experimental method is used, and an L 25 orthogonal arrays table is constructed.
The design of experiment (DOE) adopts the L16(45) orthogonal arrays table which is four levels and five factors type of factorial design.
Utilizing the Taguchi Method, the L 25 orthogonal arrays table with 25 rows is constructed for the controllable factors (Table 4).
Interestingly, the same forty-three transconjugants were positive for SGI1 tandem arrays (Table 3).
Phases obtained for these two markers by sequencing were identical to those from digital arrays (Table 1 and Table S1).
Our aCGH chips lacked a total of six genes that were, however, present on expression arrays, Table S2.
Then, we combined all four microarray datasets into a composite training set (excluding 39 outlier samples), which consisted of 239 tumor arrays (Table 1, Figure 1).
The novel candidate housekeeping genes identified in the human data set also showed stability in expression in mouse arrays (Table 3).
Therefore, a total of 745,736 bases were sequenced across the 31 arrays (21 patient arrays and 10 control arrays; Table 2).
Results based on this analysis confirmed that the correlation between MYCN binding and DNA hypermethylation extended to intergenic regions tiled on the arrays (Table 2).
Of the remaining 2,614 probes, 600 were determined to not be variable in our sample based upon hybridization to all (or none) of the arrays (Table S1).
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