Examples are presented that show the utility of this approach for studying the 3-D architecture of membrane systems and cytoskeletal arrays in intact cells.
The SEM images indicated that the nanodot structures of the used 100-nm and 200-nm nanodot arrays were intact even after a prolonged culturing of cells (Fig. 4). Figure 4 SEM images of the used nanodot arrays.
The CNNC arrays with intact shape, high optical absorption, high electrical conduction, and nice wettability to polymer are pursued for potential uses as electrodes or even absorbers in photovoltaic devices and photodetectors.
The uniqueness of S-layers resides in their periodicity and in the capability of isolated S-layer protomers to recrystallize by an intrinsic, entropy-driven process into monomolecular arrays on intact bacterial cells, in suspension, and on various surfaces or interfaces particularly on lipid films.
The fact that expression arrays on intact human TZ tissues xenografted into hormonally manipulated host mice identified many genes well known to be androgen regulated validates our approach.
Contrary to the large regular arrays on intact cells, the crystalline patches were much smaller.
We initially tried to image cytoplasmic chemoreceptor arrays in intact R. sphaeroides by ECT.
Interestingly, while transmembrane arrays remain intact upon cell lysis, presumably due to the stabilizing effect of the membrane, cytoplasmic arrays fall apart unless molecular crowding agents are added.
As discussed above, due to the substantial density and thickness of R. sphaeroides cells, we were unable to identify cytoplasmic chemoreceptor arrays in intact cells.
Furthermore, MN arrays remained intact and no damage to the MN was noted during application, wear (as evidenced by OCT, Figure 6l), or removal of MN-containing plasters.
