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Although capillary condensation is the adopted terminology to depict phase transformation in nanoconfined surfaces [29], it is also a suitable mechanism for the fabrication of needle-like nanostructure arrays based upon nanosized templates.
The multi-strain, multi-species microarray design strategy can be used to identify the presence and absence of genes in a more informative fashion than arrays based upon single genomes, or even single species.
Since the majority of currently published studies on Drosophila immune-responsive genes used the Affymetrix version 1 Drosophila Genome Arrays (based upon Berkeley Drosophila Genome Project v4.0), they could not identify all immune-regulated loci.
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Probe set enrichment was evaluated by comparing the number of significant probe sets for an individual gene to (1) number of probe sets on the array for that gene, (2) number of probe sets significantly changing, and (3) the total number of probe sets on the array based upon a binomial distribution.
Rodent oligonucleotide arrays are based upon the sequence of the B6 mouse or Brown-Norway (BN) rat strains.
It should be noted that the data generated by the Harvard arrays was based upon a combination of short range and long range PCR.
For this procedure we used all the default Affymetrix methods defined by the GEPAS 4.0 system which were implemented in the "affy" package from Bioconductor [ 74]: a) background correction by Robust Multi-array Average (RMA) expression measure [ 75], b) between array standardization based upon quantiles [ 76], and c) PM-MM adjustment using "pmonly" (just PM values are used).
CNV determination on the digital array is based upon its ability to partition DNA sequences.
The proprietary xMAP® (Multi-Analyte Profiling) technology is a suspension array platform based upon colour-encoded microspheres, more often referred to as beads [ 32].
Candidate genes were identified from the array studies based upon their fold-change between AS and control, the P-value of this difference and their potential biological relevance to AS. Candidate genes were assayed using real-time quantitative PCR-based (qPCR) predesigned TaqMan Low Density Array Cards (TLDA).
This major issue of single template comparison has been avoided in our new technique, as an array of filters based upon identified EPSCs from experimental data in the training stage provides a range of templates with variable shapes and durations that potentially match a variety of EPSCs found in experimental recordings.
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