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Fortunately, we can reduce the memory by only storing a small fraction of the O and S arrays, and calculating the rest on the fly.
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As before, we normalized the values in each of the six types of arrays and calculated their exact local Whittle estimators.
To select proteins that interact with total RNA or mRNAs, we retrieved background subtracted median signal intensities of both channels from five replicate arrays, and calculated percentile ranks from 0 to 1 for each channel and array (raw data is given in Dataset S2).
The β values were calculated by subtracking background using negative control on the array and calculating the ratio of the methylated signal intensity to the sum of both methylated and unmethylated signals plus a constant of 100.
This value is calculated by subtracting background hybridization levels obtained from negative control probes on the array and calculating the ratio of the fluorescent signal from the methylated allele (M) to the sum of the fluorescent signals from both unmethylated (U) and methylated alleles (|U|+|M|+100).
PCA could be considered as a "black box" that extracts some hidden features in the input data array and calculate new output data array that describes substances.
Scanned images obtained by the Affymetrix GeneChip Operating Software (GCOS) v1.2 were used to extract raw signal intensity values per probe set on the array and calculate detection calls (absent, marginal, or present).
This random sampling was repeated 10,000 times to approximate the null odds ratio distribution of each term on the array and calculate the significance of observed methylation enrichment.
EASE performs a statistical analysis of gene categories in a gene list relative to all genes on the array and calculates a conservative variant of the standard Fisher exact probability called the EASE score.
We analyzed DNA from mouse mammary gland epithelial cells using the Affymetrix Mouse Diversity Genotyping array (MOUSEDIVm520650) and calculated the CNAs.
We defined signature gene sets for each SqCC subtype based on the DE analysis of array data and calculated the signature scores for each subtype and each bronchial epithelial sample.
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