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The design and construction details of the array are discussed in addition to the measurement considerations required for appropriately characterizing an array when using ULOI amplifiers.
Since a large amount of hemoglobin exists in erythrocytes, several studies have shown decreased present calls, reduced accuracy, and increased variability among replicates in an Affymetrix GeneChip array when using PAXgene RNA collection technology[5], [13].
Despite the reduction of the feature number and feature size, the absolute error between real and estimated allele frequency with the 250 K array was as low as the one for the 10 K array when using Simpson's k-correction.
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For an infinite plate analytic solutions can be obtained for the total power supplied to the plate by the primary and secondary actuator arrays when using the two control strategies.
From the clear split between arrays 3 and 7, and the remaining arrays when using 2 up to 20 genes, it was concluded that arrays no. 3 and 7 had been probed with different samples than the other four arrays.
For example, when 3 treatments and a control are to be compared using 4 data points per comparison, 16 microarrays are needed for a common reference design, 12 arrays for a block design (treatment vs. control on an array), 8 when using a loop design, but only 4 with 4 dyes and the design shown in Table 4.
Table 2 shows an improvement in the correlation of RNAseq to array data when using tag counts normalized to the proportion of unique sequence in each gene.
Instructors also employed a diverse array of techniques when using facilitation strategies.
There was a strong correspondence between RNA-Seq and Agilent array transcriptome profiling when using the same samples and analysis strategy.
The use of synthetic guide RNAs is also uniquely suited to genome-scale high throughput arrayed screening, particularly when using complex phenotypic assays for functional genomics studies.
Exon arrays contain one or more probe sets per exon for each gene (287 329 core probe sets for 22 011 genes), whereas only one data point per gene is generated on HU133plus2 arrays (17 583 genes, when using the alternative.cdf based on entrezgene; Dai et al, 2005).
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