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The genomic regions to be targeted by the array were generated automatically using scripts written in the Python programming language.
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Based on all the states obtained in all time steps, a state selection array is generated then a global alignment path is determined from this array.
The output on port C to the ULN2003AN Darlington array is generated by looking up the correct step for each motor and combining the x-axis and y-axis outputs into the upper and lower nibbles of port C. Timer 2 was configured to run at 15.625 kHz by prescaling the system clock by 1024.
b Two-dimensional spot array was generated by the double cross optical grating.
First, a single-crystal TIPS-PEN nanowire array was generated on a Si substrate as active p-channels using LB-nTM.
A mock array was generated by averaging the expression of each miRNA in all samples.
To study the serological response of tic patient versus a representative panel of GAS antigens a protein array was generated by printing 102 recombinant proteins, mainly selected from the GAS SF370 M1 genome (Table S1, Supplementary Information).
Using a window of 500 bp to define the positioning of peak signals, a composite origin profile at the 5'-end of all genes present in the array was generated.
The GAS protein array was generated by spotting affinity-purified recombinant protein (0.5 mg/ml) in 4 replicates on nitrocellulose-coated slides (FAST slides, Schleicher and Schuell) with the Chipwriter Pro spotter (Biorad), resulting in spots of approximately 150 µm in diameter.
(73) The two-dimensional (2D) projection map of the dodecamer array was generated using the EMAN software as described elsewhere.
A negative control for the array was generated by PCR amplification of sonicated zebrafish liver genomic DNA.
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