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The hybridization of fluorescently labeled samples to the array were detected and quantitated with a laser confocal scanning microscope.
At least more than 27,000 genes out of 41,000 gene probes on the array were detected in each sample, and the Volcano plot of the probe data set averages of gene expression in the spinal cords of the G93A mice (n = 4) compared with the WT mice (n = 4) is shown in Fig. S7A.
140 miRs (18% of total 784 on array) were detected across the three media groups.
All of the 32 genes represented on the array were detected as expressed in all the samples analyzed.
Antisense biotin-labeled cRNA (10 μg), generated by in vitro transcription were hybridized and biotinylated probes hybridized to the array were detected using pycoerythrin labeled streptavidin.
Human antibodies reacting with expressed proteins on array were detected by Cy5-labeled anti-human IgG (Jackson ImmunoResearch Laboratories, West Grove, PA, USA).
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After the T7 phages were trapped, the fluorescent signal from an individual array was detected with a single photon counting detection system with excitation from a 532 nm laser and collection of 570 nm emitted fluorescent signal from the T7 SYTOX Orange labeled phages on the array.
Experience with multiple moving person detection has shown that the target located closest to a radar antenna array is detected with the highest probability.
In MPPT, voltage and current of the PV array are detected by voltage and current sensors at a particular sampling cycle, respectively [69].
The variation of the drain current in an OTFT in the fingertip-shaped OTFT array was detected in relation to a vertical displacement of Δ h under V GS = −30 V and V DS = −30 V.
In contrast, 43% of female-biased genes on the array are detected in females, while only 28% are detected in males.
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