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STEP 3 – When needed, the fractions of interest were subjected to a final purification step on a narrow-bore reversed-phase column (Xbridge BEH130, Waters Associates) at 40°C at a flow rate of 0.25 mL/min using the biphasic gradients described in STEP 2. All HPLC purifications were performed on a Waters HPLC system (Waters 600 pump) equipped with a photodiode array (Waters 996 PDA).
this was monitored at an absorbance of 259, 280 and 360 nm each of Rutin [ 30, 31], Catechin [ 30, 32] and Kaempferol [ 33] respectively, using a UV-detector (Photo-Diode Array, Waters Model No. 2996).
5 µl aliquots were injected onto a separation column (ACQUITY UPLC BEH C18, 1.7 µm, 2.1×100 mm reversed-phase column, Waters) with a flow rate of 0.5 ml min−1 and analysed with an Acquity Ultra Performance Liquid Chromatography (UPLC Waters) interfaced with PDA photodiode Array (Waters).
In order to study the influence of UV irradiation on surface-wettability response of the SA-coated ZnO nanoflake arrays, water contact angles are evaluated as a function of UV irradiation time in ambient conditions.
Analyses were performed using a 2690 Waters Separations Module (Waters, Milford, MA, USA) equipped with a 996 Photodiode Array Detector (Waters) with a Waters YMC ODS-AM (Waters, Milford, MA, USA) narrow bore column (100 × 2 mm i.d.; 3 μM particle size).
TNA, TiO2 nano-tube array; DI-water, deionized water; XRD, X-ray diffraction; SEM, scanning electron microscopy.
LC/TOF-MS analyses were carried out using an Acquity Ultra Performance liquid chromatography (UPLC) system (Waters) linked simultaneously to both a PDA 2996 photo diode array detector (Waters) and a Waters LCT premier XE mass spectrometry (Waters), equipped with a electrospray ionization (ESI) source.
Carotenoids were separated by HPLC (Waters, Barcelona, Spain) on a reversed-phase C18 column (Waters Spherisorb ODS1, 4.6 x 250 mm, 5 μm particle size, Milford, Massachusetts, USA) and detected with a Photodiode Array Detector (Waters 996, Waters, Barcelona, Spain), following the method of García-Plazaola and Becerril [ 40, 41].
Areinvented ITT, which began life in the telegraph business, is now centered on defense gadgets like radar-jamming systems and night-vision goggles for military use, along with an array of water pumps, aerators and filters for water-treatment plants.
The HPLC system consisted of a 515 HPLC binary pump (Waters, USA), a Rheodyne 7725i manual injector (Waters, USA), Photodiode Array Detector (Waters, USA), and the operating software Empower Node 2054.
Mass spectrometry was performed using ESI/MS (Waters 2998 photodiode array detector, Waters LCT premier XE mass spectrometry).
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