PRL-arrays were visualized as bright foci of nuclear fluorescence in GFP-ER-expressing cells (Figure 1A, arrows), These foci co-localize with the integrated PRL reporter chromatin by DNA and RNA fluorescence in situ hybridization (FISH) [19].
After each spotting runs, samples of printed arrays were visualized with a fluorescent dye to control evenness of DNA deposition and spot morphology.
Nuclear envelope was visualized by immunofluorescence (IF) using nuclear pore antibody, LacO arrays were visualized by green fluorescent protein (GFP) fluorescence of GFP-LacI introduced from the HS > GAL4 UAS > Cap-H2 and HS > GAL4 line.
Cytokine array luminesence was visualized with ECL substrate and autoradiography.
The variation or reproducibility of gene expression between arrays of different groups was visualized and assessed by creating scatterplots, which are shown in Figure 2.
CEN3 was visualized by adjacent insertion of a tet operator array that was bound by Tet repressors fused with green fluorescent protein (GFP) [10], and therefore CEN3 was visualized as a small GFP dot.
The distribution of transcriptional signals along the genome coordinate was visualized with the In Silico Molecular Cloning program, Array Edition (In Silico Biology).
The distribution of protein-binding signals along the genome coordinate was visualized with the In Silico Molecular Cloning program, Array Edition (In Silico Biology).
The number of juxtaposed CtBP2 and GluA2 puncta or CtBP2/GluA2 puncta and tdTomato was visualized and counted from these miniature image arrays.
EGFP was visualized directly.
