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In the second method, porous anodization was performed on an Al thin film on Si and a SiO2 nano-island array was subsequently formed electrochemically.
The output performance of the LED array was subsequently recorded in terms of the change in uniformity of illuminance, LED efficacy, and radiant flux.
The array was subsequently deemed a loss.
The array was subsequently crossed into all other backgrounds; genotypes were confirmed by single worm PCR.
The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythrin (SAPE) [18] and scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
Each array was subsequently scanned using an Agilent DNA microarray scanner, and images were processed using the GenePix Pro software (Molecular Devices, Sunnyvale, CA, USA).
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Normalized datasets for each array were subsequently loaded into GeneSpring software (v.7·3·1; Agilent Technologies) for analysis.
Promoter regions of both groups of differentially expressed genes and all RefSeq genes on the array were subsequently screened for the presence of all unique motifs using MAST.
The resulting multiplex Ab-array was subsequently applied for a large-scale evaluation study using 680 serum samples obtained as part of the national DS screening program.
The combined set of arrays was subsequently quantile normalized.
Variation in expression between arrays was subsequently clustered by wing, forewings were further separated by morph, and sections of forewings from the same individual tended to cluster within morph.
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