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The resulting data collected from each array was submitted to the Stanford Microarray Database (SMD, ) [ 34].
Response: The sequence resulted from inverse PCR product, showing the circular form of the gcu14-blaGES-1-aacA4 gcu14-blaGES-1-aacA4 gcu14-blaGES-1-aacA4 gcu14-blaGES-1-aacA47.
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The initial proposals from Celtic Array were submitted to the UK government in the summer.
The images were quantified using GenePix Pro Image Analysis 5. Background subtracted intensities in the red and green channel of non-flagged spots on each array were submitted to quantile normalization [21] and transformed using glog transformation [22].
Only trials with clear echoes from the target and the array were submitted to further analysis.
A total of 97 arrays were submitted (60 experiments done with BC2 and 37 experiments performed with BC91).
Fragmented cRNAs and GeneChip arrays were submitted to the Gene Expression Center at UW-Madison for hybridization, washing and scanning following standard manufacturer's protocols.
Raw Cy5 intensities, raw Cy3 intensities, LOESS-normalized log2 Cy5:Cy3 ratios, and LOESS-normalized log2 average intensities (A), as well as original GPR files for all arrays, were submitted to the National Center for Biotechnology Information (NCBI)'s Gene Expression Omnibus (GEO) [GSE19585].
The array-CGH data was submitted to NCBI GEO (accession number, GSE22635).
Array comparative genomic hybridization microarray data was submitted to Gene Expression Omnibus (GEO) Accession No. GSE27636.
The generated array data set complies with MIAME [ 21] and was submitted to NCBI's Gene Expression Omnibus (GEO), accession number GSE54958.
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