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The mean intensity for each array was scaled to 100.
The signal of each array was scaled to the intensity value of specific constant controls.
In order to perform interarray comparisons, the data for each array was scaled to a target intensity of 1,500 using Affymetrix Microarray Suite software.
The overall intensity of all probe sets of each array was scaled to 500 to ensure equal hybridization intensity; each probe set was assigned "P", "A" or "M" and a p value from the algorithm in GCOS.
First, the 18-zone mfVEP array was scaled to the fundus photograph grading sheet.
The 103 hexagon mfERG array was scaled and superimposed onto the 18-zone mfVEP array.
Similar(51)
All hybridized chips met standard quality control criteria, and mean fluorescence values of each array were scaled to a mean intensity of 500.
The raw intensities of each array were scaled to the array median.
The log2 perfect match (PM) probe signals for each array were scaled such that the average for each array was the same as that for wild type.
In a second normalisation step the M-values from each array were scaled to equalise the median absolute value across all arrays to account for technical biases between replicate hybridisations [ 52].
Overall intensities of all probe sets of each array were scaled to 500 to guarantee that hybridization intensity of all arrays was equivalent, and each probe set was assigned a "P" (present), "A" (absent) or "M" (missing) value and p-value from algorithms in GCOS.
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