Signals from the antenna array were collected using a Fraunhofer multiple RF front-end, which can collect digital samples from all the antennas simultaneously.
Fluorescent intensity data from each array were collected using a GenePix 4000A scanner (Axon Instruments).
In a typical experiment, signals of the GNP sensor array were collected for 5 min in vacuum, then for 5 min during breath exposure, and then again for 5 min in vacuum.
Image files on all arrays were collected with a ScanArray 4000 scanner (Packard BioScience, Billerica, MA, USA).
Image files on all arrays were collected before hybridization using excitation and emission spectra specific for fluorescein using a ScanArray 5000 (GSI Lumonics, Billerica, MA).
The CNVs detected with both kinds of arrays were collected together, defined by the distal ends of the first and last probe altered.
Image files on all arrays were collected after blocking (fluorescein), and again after hybridization (Cy3 and Cy5) with a ScanArray 5000 (GSI Lumonics, Billerica, MA).
In addition, breastmilk, saliva (mother and infant), and infant blood for peptides/cytokines/gene arrays are collected and stool is sampled to assess the gut microbiome and markers of inflammation.
To this end SNP array data was collected from tumor cells in a microdissected lung cancer sample and from lung cancer tissue outside of the tumor from the same patient.
These data manipulations were necessary because the oligo-nucleotide array data was collected as the intensity ratio between the experimental sample and a common reference, while the photolithography data was collected as the total signal intensity at each spot.
