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Scrambling of samples into multiple racks can easily be achieved with the Sample Array Tube Handler platform.
The Sample Array Tube Handler (Thermo Scientific, San José, CA, USA) is specifically designed to be used with 384-tube plates.
In order to investigate the rigidity and stability of the Sample Array Tube Handler, we performed repeated picking and sorting steps in the 384 rack system.
By this procedure, we are able to efficiently handle both biofluids and solubilized tissue extracts in the Sample Array Tube Handler system.
Consequently, the CSV-file generated by the Sample Array Tube Handler platform for each of 384 plates is automatically fed into the database through the laboratory information management system (LIMS).
None of the sample tubes containing plasma and erythrocyte blood fractions showed any trace of leakage, which was considered a confirmation of the excellent performance of the Sample Array Tube Handler.
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Arrays were spotted with six-fold redundancy on surface-coated glass using Alere Technologies spotting machines and assembled into array tubes as described previously [ 17, 18].
Five microliters (5 μl) of the PCR product proceeded in hybridization on the specific Array Tubes according the instruction manual (Biogenomica Spain) on the ATS Workstation (CLONDIAG Chip Technologies GMBH).
Most importantly, the structure control in TNT array and tube levels can be performed separately.
The wall temperatures in the centre core area (tube array region) are relatively uniform, whereas a huge temperature gradient is measured in the peripheral area.
"This is actually quite complicated," Mr. Grucci said, pointing to the precise array of tubes and wiring on the barges.
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