Exact(2)
With each array tiling >21 Mb, a total of >1.2 Gb of sequence was subsequently statistically assessed for DNA methylation status.
To determine if a gross chromosomal rearrangement was responsible for the mouse PPCD1 phenotype, comparative genome hybridization was carried out on DNA from wild-type and PPCD1 animals using the Nimblegen 395 K Whole Genome 8 Array Tiling Set, which contains 385,000 50-75mer probes covering mouse Chromosome 2 from 124 Mbp to 181.9 Mbp at an average spacing of 656 bp.
Similar(58)
FIRMA has been developed for Affymetrix exon arrays, but could in principle be extended to other exon arrays, tiling arrays or splice junction arrays.
Both Cy3- and Cy5-labeled DNA were hybridized to high-density arrays tiling through ENCODE regions with 50-mer oligonucleotides across nonrepetitive genomic regions.
BJC supervised the AMP assay, tiling array, discussed results and contributed to the manuscript.
The latest generations of microarrays, like exon arrays or tiling arrays, address transcript expression analysis by an increased number of probes and respective selection of probe sequences.
The genome tiling array data is derived from phase 2 of Affymetrix tiling array studies [ 10].
Thus the cost of tiling array is much higher and use of tiling arrays for routine measurements of NAT expression in a large number of samples is difficult.
We successfully verified all sORFs detected by tiling array analysis using RT-PCR.
We thank Zhi John Lu and Ashish Agarwal for performing initial analyses of tiling array data.
EDW performed tiling array experiments.
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