In T. reesei and A. niger, transcript abundance from genes encoding GH family proteins 3, 5, 6, 7, 11, 30, 31 and 67 (glucosidases, cellobiohydrolases, xylanases and glucuronidases) and AA family 9 (copper oxidoreductases) was highest for both organisms (Table 1) indicating that both species use a similar array of GHs for wheat straw deconstruction.
This approach detected rye-origin NORs as condensed knobs proximal to the nucleoli, suggesting their low transcriptional activity, in accord with the silencing of rye origin rRNA gene arrays in the presence of wheat genome (i.e. nucleolar dominance, [21]).
A total of 140 barc, 1 cfa, 34 cfd, 18 gdm, 109 gwm, 4 hbd, 15 hbe, 41 hbg, and 60 wmc loci were used for chromosomal assignment of wheat array-based markers (WABMs) developed in this study.
To analyze the possible association between genetic variation and transcriptional pattern, the sequences were subject to BLASTn against the sequences (60 nt) of differentially expressed probes of wheat cDNA array in our previous study exploring the transcriptomic difference between SR3 and JN177 seedlings under the control and after exposure to NaCl and PEG treatments [ 58].
Extensive research has been conducted to investigate protein synthesis and accumulation, and a number of proteomic studies have focused on identifying the array of proteins in developing wheat grains.
Using bi-parental genetic populations, a total of 41 DArT markers by Akbari et al. [ 31], and 55 markers by Wang et al. [ 32] were mapped on D-genome of wheat using DArT array.
Trade ministers from more than 140 nations meeting in Qatar agreed on an agenda to topple tariffs and liberalize trade on an array of items as diverse as wheat and insurance.
If established as a large-scale functional assay system, transient expression could be utilized to enable high-throughput loss-of-function screening of a diverse array of PST effector candidates in wheat.
An expanded version of the WHEAT 2.6 DArT array was used where coverage for the D-genome of wheat was increased by adding markers from a PstI(TaqI) genomic representation of D-genome enriched DNA fractions.
Empirically defined filtering criteria were used to facilitate a robust integration of 17 deposited microarray experiments (148 arrays) of Arabidopsis, rice, wheat and barley.
