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The sensor is based on an array of total internal reflection point sensors multiplexed on a single fiber by optical couplers and simultaneously interrogated by an Optical Time Domain Reflectometry approach.
Microarray analyses using the Affymetrix Human Genome U133 Plus 2.0 array of total PBMCs revealed a molecular signature comprised of genes that are involved in innate sensing of viruses and antiviral immunity, in most of the vaccines.
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Superarray quantitative PCR arrays of total RNA extracts from HMVEC treated with either arsenical for 24 hr demonstrated that the arsenicals differentially affect expression of a limited set of 9 of 84 inducible genes measured.
In order to ascertain the cancer gene pathways modulated by OPNc overexpression in the OvCar-3 and PC-3 cell lines, we performed a qRT-PCR Array analysis of total RNA obtained from OPNc-overexpressing cells, compared to cells transfected with the EV control clone.
A plastic scintillator array of 3.4 m2 total area for the detection of neutrons in medium-energy nucleon nucleon reactions was constructed and built.
Micro-array analyses of total RNA extracted from biopsy samples at 48 H and 14 D uncovered 575 and 3,128 probes, respectively, which were significantly altered during immobilization.
For each array, 100 ng of total RNA was labelled for hybridization to a GeneChip Human Gene 1.0 ST Array (Affymetrix) according to the manufacturer's protocol.
For each cDNA array, 15 µg of total RNA were used for reverse transcription and indirect labeled with the fluorescent dyes.
For each array, 10 μg of total RNA were converted to cDNA.
For each oligo array, 35 μg of total RNA was used to make Cy-5 or Cy-3 labeled cDNA.
In brief, for each array, 2.5 μg of total RNA were reverse-transcribed with MMLV reverse transcriptase in the presence of 35 μCi of [α-P] dATP.
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