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order, which is an array of operators ordered according to its mobility over pipeline stages; .
1. asap, which is an array of operators with the corresponding pipeline stage using the ASAP algorithm; 2.
optimalColor, which is the optimal pipeline schedule as an array of operators with the corresponding pipeline stage; 3.
alap, which is an array of operators with the corresponding pipeline stage using the ALAP algorithm; 3.
asap, which is an array of operators with the corresponding pipeline stage using the ASAP algorithm; alap, which is an array of operators with the corresponding pipeline stage using the ALAP algorithm; order, which is an array of operators ordered according to its mobility over pipeline stages; and generates the following output: 1. pipelineCount, which is the number of generated pipelines; 2.
pipelineCount, which is the number of generated pipelines; optimalColor, which is the optimal pipeline schedule as an array of operators with the corresponding pipeline stage; minRegWidth, which is the minimum total register width of the optimal pipeline schedule.
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For live imaging, yeast strain GA-1459 was used, which has TEL VIR tagged with four LexA operators (LexO) and an array of Lac operators (LacO), and expresses GFP-LacI to visualize the LacO and GFP-Nup49 to tag the nuclear periphery [ 93].
We monitored the localization of a native telomere in living cells by microscopy, using a strain that has telomere VIR tagged with an array of Lac operators bound by green fluorescent protein (GFP -LacI and telomeric LexA operators.
The CMV promoter is also preceded by an array of tetracycline operators.
This strain has CDC20 under control of the MET promoter, expresses the GFP-Tet repressor and has CENIV "GFP-marked" with an array of Tet operators, and has its SPBs marked with Spc42-tomato.
In addition, we found that ybp2Δ cells did not exhibit sister chromatid cohesion defects by the cohesion assay, using a strain an array of lactose operators integrated at the TRP1 locus, 12 kb from the CEN of chromosome IV and expressing a GFP-lactose repressor (GFP-lacI) [34], [35] (Supplemental Figure S4).
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