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The array of different biological processes that can be regulated by Rho GTPases coupled with the significant degree of coordination that occurs between them makes an analysis of Rho GTPase signaling pathways a challenge.
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The accumulated GO annotations have been shown to be increasingly important in an array of different areas of biological research ranging from high-throughput omics data analysis to the detailed study of mechanisms of developmental biology (3 6).
However, since there were not sufficient amounts of the Universal RNA reference remaining, triplicate arrays using different biological replicates of the AR97Q males (experimental, Cy5) were directly compared with their WT brothers (reference, Cy3).
Two independent experiments were performed to each array using two different biological samples and dye swap analysis.
The analysis of the tissue extracts has the potential to provide for identification of molecular biomarkers associated with any biological process in a wide array of different tissue types.
It also comes in an array of different languages.
The PCA plot of individual qPCR results showed a sample clustering similar to the array results, corroborating the different biological sources of the two sample groups (Fig. 2).
Furthermore, the chip is composed of an array of individual detector elements, each of which could be devoted to the detection of a different biological agent for multiplexed detection.
Consequently, the commonly used quality control method of applying correlation analysis on technical replicates can be adopted for assessing array performance based on different biological samples using tERCs.
Ten genes were chosen on the basis of the microarray gene expression data that showed low coefficients of variation across all arrays, and also had different biological functions to avoid co-regulated genes.
Array hybridizations were performed at least twice per each time point using different biological replicates of plant material.
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