Muscles of the embryonic zebrafish undergo dynamic changes in ECM composition (Henry et al., 2001; Crawford et al., 2003; Snow and Henry, 2009; Sen et al., 2011), in which early high levels of Fn are replaced by Lams and Cols and later by an orthogonal array of collagen fibrils (Kannus, 2000; Câmara-Pereira et al., 2009; Charvet et al., 2013).
See related research by Montfort et al and Young et al Proficient operativity of articular cartilage is effectively maintained via the specialized qualities of multiple macro-molecular components, including a notable array of collagens and proteoglycans.
In this investigation we have used the simple technique of spin-coating to produce highly aligned arrays of collagen fibrils.
Nonetheless, tissue engineering efforts, which have been focused on producing the fundamental functional component of a cornea (organized alternating arrays of collagen or "lamellae"), may have already provided valuable new insights and tools relevant to development, growth, remodeling and pathologies associated with connective tissue in general.
Bone is a mineralized tissue with an ordered composite structure consisting of arrays of collagen fibers with inter-grown carbonated hydroxyapatite crystals.
Close examination revealed fibers of fibronectin and collagen I radiating away from the xenograft, typical of the arrays of collagen fibers detected around breast tumors that are associated with files of invading cells (Provenzano et al., 2006).
In addition, we found high reflectivity in the UV spectrum, which might be produced by a series of mechanisms such as coherent scattering on arrays of collagen fibers in the dermis [ 28] or incoherent scattering [ 29] on, for example, melanosomes or guanine crystals.
A number of these genes have similarity with previously characterised streptococcal proteins; SUB0145, encodes a lactoferrin binding protein (Lbp) [ 52] which displays homology to fibrinogen-binding/M-like proteins, and SUB1095 shows similarity to an array of SclB collagen-like surface proteins of S. pyogenes [ 53].
Accordingly, we found in our array analysis the upregulation of collagen type I and IV in immunized rat hearts.
We show that second harmonic generation (SHG), third harmonic generation (THG) and Coherent anti-Stokes Raman scattering (CARS) microscopy techniques provide complementary information about the sub-micron architecture of collagen arrays.
To assess whether mifepristone impairs the capacity of cancer cells to adhere to extracellular matrix, cells were pre-treated for 72 h with or without a cytostatic concentration of mifepristone, trypsinized, and re-plated in commercially available plates that had been pre-coated with an array of extracellular matrix proteins including fibronectin, collagen I, collagen IV, laminin or fibrinogen.
