In the second method, porous anodization was performed on an Al thin film on Si and a SiO2 nano-island array was subsequently formed electrochemically.
The output performance of the LED array was subsequently recorded in terms of the change in uniformity of illuminance, LED efficacy, and radiant flux.
The array was subsequently deemed a loss.
The array was subsequently crossed into all other backgrounds; genotypes were confirmed by single worm PCR.
Each array was subsequently scanned using an Agilent DNA microarray scanner, and images were processed using the GenePix Pro software (Molecular Devices, Sunnyvale, CA, USA).
The hybridized probe array was subsequently stained with fluorescent protein streptavidin-phycoerythrin (SAPE) [18] and scanned with a GeneArray scanner at the excitation wavelength of 488 nm.
Normalized datasets for each array were subsequently loaded into GeneSpring software (v.7·3·1; Agilent Technologies) for analysis.
Promoter regions of both groups of differentially expressed genes and all RefSeq genes on the array were subsequently screened for the presence of all unique motifs using MAST.
Each GPCR array was subsequently run on a 7900HT Fast Real-Time PCR System (andlied Biosysthes) and the resulting data was analyzed using the SDS/Relative Quantification Manager v.1.2 and the DataAssist v.3.0 software packages (both Applied Biosystems).
The arrays were subsequently washed and stained.
Arrays were subsequently washed, stained and scanned using an Affymetrix GeneChip®-related software.
