Exact(6)
Simulations have been carried out at two intermediate Re and the lower Re results exhibited stronger array interactions.
By combining S2 and S3, the Gene × Array interactions occurring specifically on S2 and S3 are pooled.
For the analysis reported in the text we used a cutoff of arrayscore < 0.6 to identify high-confidence array interactions.
The remaining 14.9 % variance in the VSG interaction still contains the Gene × Array interactions for S1 (7.8 %) and sampling differences (7.1 %; Table 2).
The -uracil selection data allowed us to compare 237 pairs, and we found that 7 strong interactions observed by Y2H were not seen on the arrays, and 23 strong array interactions were not detected by Y2H, for an overall discrepancy of ∼13%.
On the basis of comparisons of 235 pairs (see Methods) under -histidine selection, 12 strong interactions observed by Y2H were not seen on the arrays and 12 strong array interactions were not detected by Y2H, for an overall discrepancy of ∼10%.
Similar(54)
Peptide array interaction assays.
The reference thus made it possible to take into account any eventual "spot × array" interaction.
The reference allowed thus to take into account an eventual "spot × array" interaction.
For example, from peptide array interaction studies of PDE4B and PDE4D with DISC1, it was noted that the PDE4 family possessed both common binding sites and also isoform-specific ones (Table 1).
Our previous results, based on peptide-array interactions, suggested that the cohesin loader makes multiple contacts with cohesin around its ring circumference, including Psc3 and the SMC hinge (Murayama and Uhlmann, 2014).
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