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Several cDNAs on the array correspond to the same gene and to reduce redundancy, a sequence based clustering was made as follows.
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For example, three probes on the array corresponded to the Os11g0216900 gene (IDI2).
The nanodot array corresponds to the configuration of the self-ordered pore array in the anodic alumina mask, as shown in Fig. 2a.
In ICA, the third dimension K of the three-way array corresponds to the number of covariance matrices at different lags, or the number of matrix slices derived from a cumulant array.
Each pointer array corresponds to a possible base 4 value of a 13-nt sequence, and the number of elements in each array corresponds to the number of occurrences of a given 13-nt sequence in the genome.
The values stored in the array corresponded to the number of times that a particular triplet appeared in one ORFeome.
These arrays correspond to the conditions "B-cell + SIMDM exposure = 0 minutes" vs. "Spleen".
To find matches to a new sequence, as in Algorithm 5, we need also the stored arrays u k and v k, in order to evaluate the extension function These arrays correspond to the information stored in the index described by Ferragina and Manzini (2000), commonly known as the FM-index.
In order to evaluate the biological power of this tool we included a pool of RNAs representing virtually all tissues and developmental stages (RNA cocktail) to determine the extent to which the oligos selected for the array correspond to transcribed sequences that can be visualized with this technology.
Eighty three percent of oligos on the NSF 45K array correspond to a single transcript (gene model), and the remaining oligos represent genes associated with multiple transcripts [23].
Since most of the genes represented in this array correspond to genes identified from fed nymphs and adults, it was not surprising that most of the genes were not expressed or expressed at lower levels in 24 h fed salivary glands compared to that in 66 h fed salivary glands.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com