Film mammograms of the medio-lateral oblique view were digitized by using an Array 2905HD Laser Film Digitizer (Array Corporation, Tokyo, Japan), which covers a range of 0 to 4.7 optical density.
Film mammograms of the medio-lateral oblique view were digitized using an Array 2905HD Laser Film Digitizer (Array Corporation, Tokyo, Japan), which covers a range of 0 to 4.7 optical density.
Film mammograms of the medio-lateral oblique view were digitised using an Array 2905HD Laser Film Digitizer (Array Corporation, Tokyo, Japan), which covers a range of 0 to 4.7 optical density.
Screen-film mammograms were digitized on the Array 2905 laser digitizer (Array Corporation, Roden, The Netherlands), which has 50-μm (limiting) pixel spacing with 12-bit grayscale bit depth.
Alternatively, RT2 Profiler™ PCR arrays were used to monitor 84 mouse inflammatory cytokine and receptor genes with build in house hold genes (Super Array Bioscience Corporation, MD, USA).
5 ng of RNA were used to synthesize the cDNA for the 96.96 dynamic array (Fluidigm Corporation, CA, USA).
Analysis was carried out using the 48.48 dynamic array (Fluidigm Corporation, CA, USA) and a Biomark platform following the manufacturer's protocol.
qPCR was carried out using the 48.48 or 96.96 dynamic array (Fluidigm Corporation, CA, USA) following the manufacturer's protocol [ 19].
To knock down TXNIP expression, we performed four independent transfections with four different plasmids carrying a double-stranded oligonucleotide targeted to different regions of the rat TXNIP mRNA (Super Array Bioscience Corporation, Frederick, MD, USA), as recently described.
The high-throughput microfluidic qPCR assays were performed at the Genotoul Platform (http://genomique.genotoul.fr/) using the BioMark® 96:96 Dynamic Array (Fluidigm Corporation, San Francisco, CA, USA), as described in Cassan-Wang et al. [ 33].
The "promer" was tested with an equimolar mixture of cDNA from all the tissues (except fiber, which are included in the cortical peels), if it did not work new primers were designed, if the new primers did not work, it was presumed to be not expressed and the primers were used regardless, and run in the 96.96 dynamic array (Fluidigm Corporation, CA, USA).
