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In addition, a number of studies have used the same set of samples obtained from Stanley Array Collection to examine various pathological markers in schizophrenia and bipolar disorder [28]-[30] [28]-[30]
RNA samples from postmortem brain specimens, Brodmann's area 46 (in the DLPFC), from 35 individuals with schizophrenia, 31 individuals with bipolar disorder, and 34 psychiatrically normal controls were obtained from the Stanley Array Collection of the Stanley Brain Collection.
The second, the Array Collection, consists of 105 individual subjects, with multiple brain regions per subject.
Nevertheless, we included another 4 studies that used the Array Collection with the same dorsolateral prefrontal cortex (BA46).
For the current analysis, the subjects in the Neuropathology Consortium and the Array Collection were divided into two groups.
In contrast to the Neuropathology Consortium, the SMRI performed the RNA extraction for the Array Collection tissues.
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The 32K bacterial artificial chromosome (BAC) re-array collection (CHORI) tiling path aCGH platform was constructed at the Breakthrough Breast Cancer Research Centre, as described previously [ 33, 39].
Although only a single allele was validated by linkage and included in the arrayed collection, the other candidates are available on request for specific genes if an investigator is interested in an allelic series.
In summary, the yeast synthetic genetic array protocol (Tong et al. 2001) was coupled with a high-content microscopy platform to image an arrayed collection of 4144 arrayed strains carrying a C-terminal fusion of GFP to each ORF (Huh et al. 2003) and expressing a tdTomato fluorescent protein from the constitutive RPL39 promoter.
The Oncomine™ database is an online collection of microarrays from various sources, usually cancer-related, and contains many "multi-arrays" (collections of analyzed microarrays, in a single study).
The procedure described here employs full-length cDNA expression libraries derived from a given tissue or cell line, which brings advantages and disadvantages, compared to arrayed collections of open reading frames (ORFs).
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