Array hybridization was performed by Exiqon.
Sample preparation and array hybridization were performed as described [42].
Array hybridization and scanning was performed as described previously [29].
Array hybridization, washing, and scanning followed standard Affymetrix protocols.
Each array hybridization was replicated using dye-swap.
Array hybridization and washing were performed as described previously [6].
The dynamic range of read frequencies was two orders of magnitude greater than array hybridization (log10 dynamic range DTE 4.37; log10 dynamic range array hybridization 2.33; Fig. 2).
We also believed that the optimal probes should emerge and prevail from array hybridization results.
Array hybridization signals are transformed and often normalized prior to evaluation of inter-sample differences.
The RNA used was identical to that used as targets for array hybridization.
4 6 ug of DNA was obtained and Roche Nimblegen performed array hybridization, washing and scanning.
