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Using a radioactively tagged DNA strand, Jeffreys could scan the genome for a unique arrangement of sequences comprising a genetic "fingerprint".
Perhaps most surprising, the composition and arrangement of sequences in centromeres have been found to be hyper-variable, primarily by the process of unequal homologous recombination [61, 63, 65].
However, the arrangement of sequences on chromosome 8 is more complex.
We compared the genome-wide arrangement of sequences and the constitution of the 24 ancestral genomic blocks (A X) of Brassicaceae in B. juncea, B. rapa and B. napus.
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The unusual arrangement of sequence in this structure was detectable in short-read sequencing as it led to an apparently structure-mediated loss of sequence during contig generation.
We designed the Stitch algorithm to use alignments of query consensus genome maps to reference in silico maps in order to predict the higher order arrangement of sequence scaffolds (Fig. 3).
The arrangement of sequence contigs NW_931635.1, NW_931636.1, and NW_931634.1 observed in the segment between 15 and 20 Mb on the sequence assembly (comprising the loci SPP1, IBSP, ABCG2, BMS382, HERC3, and FAM13A1) is in contrast not only to our RH map but also to published results based on BAC contigs [ 14, 16] as we discussed above when comparing the existing RH maps.
Using different sequence distance measures and visualization methods we describe the arrangement of the sequences in sequence space.
Loci were considered to exhibit size homoplasy if any single fragment size corresponded to two or more unique arrangements of sequence gaps (Fig. 1B vs. 1C).
On the one hand, we find that the arrangement of the sequences imparts one of the reasons for the inaccuracy of sequence-based methods for coreceptor prediction.
When they are joined, the arrangement of their sequences determines how each human differs from all others and from all other living beings.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com