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When comparing the two parasitic forms in an effort to discover a vaccine target, the researchers found that much of the difference arose from the expression of a peculiar genetic sequence known as a retrotransposon.
To confirm that the GFP fluorescence arose from the expression of the expected fusion protein, D2-GFP, proteins from lysates of transformed E. coli were resolved by SDS-PAGE, blotted, and probed with either a monoclonal antibody that recognised the DENV capsid protein [13] (Figure 1C) or one that recognised GFP (Figure 1D).
These RNAs arose from the expression of convergent genes, whereby the transcription of one or both genes extended beyond its respective coding sequence into the downstream coding regions (Additional file 1: Table S1).
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As SMB plants are distributed parameter systems (DPS) with nonlinearity arising from the expression of the adsorption isotherms, classical numerical methods for the solution of DPS are computationally demanding for MPC purposes.
Resistance may also arise from the expression of proteins underlying a specific drug's mechanism of action.
Proteomics is analogous to transcriptomics the global analysis of mRNA transcripts that arise from the expression of genes in the genome although the former is considerably more complicated.
They interpreted this as being due to intermolecular epitope spreading arising from the expression of citrullinated proteins as neoantigens in inflamed tissue.
Bacterial resistance to tetracycline arises from the expression of any one of at least 23 known tetracycline transporters, which couple export of tetracycline to the influx of protons down their electrochemical gradient [1].
Inbreeding depression refers to the reduction in offspring fitness caused by matings between related individuals and arises from the expression of recessive deleterious alleles in homozygotes or reduced frequency of heterozygote genotypes with superior fitness (e.g. [ 1, 6]).
Additionally, this dimerization was not observed by size-exclusion chromatography, although there was a contaminant protein with a molecular weight of ∼75 kDa present arising from the expression in E. coli.
It is essential that any differences that arise from the expression of such markers in trials should be solely attributable to the effect of treatment with the drug and not due to potential artefacts such as those caused by delays to tissue fixation in different sample types.
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