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OH-specific phenyl-hydroxylation products of aromatic substrates have been employed [9].
Its feasibility was confirmed for a series of model aromatic substrates.
P. putida was more effective than P. oleovorans for producing polyesters from these aromatic substrates.
CYP102A2 was subjected to error-prone PCR to generate mutants with enhanced activity with fatty acids and other aromatic substrates.
These variations would also explain differences found for the oxidation of some high redox potential aromatic substrates.
Spectroscopic investigations have confirmed the existence of some postulated intermediate species for the hydroxylation of aromatic substrates.
More insight into the binding mode of reduced FAD and aromatic substrates is of utmost importance for understanding the catalytic potential and enantioselectivity of styrene monooxygenases.
LiP oxidizes aromatic rings moderately activated by electron donating substitutes; in contrast, common peroxidases participate in the catalysis of aromatic substrates highly activated (ammine, hydroxyl, etc).
The cells performed surprisingly even in an 80% (v/v) DES-containing system for a broad range of aromatic substrates (Fig. 10).
Pseudomonas putida KT2442 (pSPM01) harboring TDO genes could effectively biotransform a wide-range of aromatic substrates into their cis-diols products.
Comparison of all variant activities on each substrate indicated different binding modes for the three aromatic substrates, supported by computational docking.
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