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Recent advances provide rapid pixel-by-pixel correction for matrix and absorption effects in different (mineral) compositions across the image area to yield true quantitative images.
A random list of numbers (1 64) was generated on Microsoft Excel and the number of mitochondria/mtDNA nucleoids was counted in eight of the corresponding ROI number for each frame and averaged over the area to yield density/µm2.
A random list of squares was generated for sampling; the mitochondria in each square were counted and divided by the area to yield density in units of mitochondria/µm2 (Figure 2B).
For segmental nerves, UAS-mtGFP driven by D42-Gal4 was imaged in the proximal, medial and distal regions of the segmental nerves; the regions were divided into 100 µm2 bins and total mitochondria counted and averaged over that area to yield mitochondria/µm2 (Figure 4A and B).
Integrated fluorescence values were divided by the corresponding ROI area to yield an integrated fluorescence density for each stereocilia tip.
Although there is only limited evidence for a strict relationship between yield and quality [ 34], in this study the effectiveness of thinning reflected the suboptimal leaf area to yield ratio of control vines (0.6 m/kg).
Vessel wall area was calculated from the difference between these two contours, and then normalised to external vessel area to yield a plaque index (PI), as previously described [22,23].
The average of these distances was then standardized by wing area to yield S1. Trait S2 is calculated as, where |x-y| denotes distance from point x to point y along the model curve that connects them.
High crop level slightly delayed harvest date around three days, but grapes from both thinned and unthinned vines reached similar sugar concentrations in all seasons except when leaf area-to-yield ratio was lower than 1 m2 kg−1, where sugar berry concentration was below 21 °Brix.
The total SHG signal intensity values thus generated were normalized by the cropped collagen area (μm) to yield a mean collagen density value.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com