In vivo high-resolution SPECT findings revealed, however, that peptide binding was not consistent in all areas of the tumor (Fig. 1, Additional file 2: Figure S2).
On a series of obtained tomographic images, foci of pathological accumulation of the radiopharmaceutical agent in solid areas of the tumor were identified.
They are attracted to hypoxic and necrotic areas of the tumor and are believed to mediate chronic inflammation, apoptosis resistance, and neovascularization, i.e., to ensure tumor survival and propagation.
Clearly, functional imaging of the tumor vasculature revealed that this is a simplified perception and that contrast agent build-up in these tumors was not uniform in all areas of the tumor.
As the test results for each histological area (in units of pixels) may or may not match that area's actual viability status, true positive was defined as the viable areas of the tumor on the histological image that were correctly identified as viable by the GTA (Fig. 4c 5), and true negative was defined as the necrotic areas of the tumor that were correctly identified as necrotic (Fig. 4c 1 + 2).
The lesions characteristically had a multipatterned architecture with tumor cells focally assuming a lacelike/sievelike growth pattern in the more stroma-rich areas of the tumor and a vague fascicular growth pattern in the more cellular foci.
TAMs localize to the invasive areas of the tumor where they secrete a variety of cytokines and proteases involved in tumor cell invasion and metastasis [6], [7].
Pictures of 6 areas of the tumor (2 from the apex, 2 from the base, and 2 from the center) were taken for quantification.
We have detected macroscopic spatial assembly integration between the spiral vascular assembly in the dorsal serous areas of the tumor (Figs. 1C, 2B) and the ventral cut surface areas where the geometric complexes were located (Figs. 3A, 3B).
Furthermore, fluid isolated from necrotic areas of the tumor will likely lead to leakage of intracellular fluid with low Na+ and creatinine to the microenvironment, and thus result in reduced tumor fluid:plasma ratios for these two extracellular markers.
