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On non-Alexa Fluor RNA arrays, corner areas of the array were marked with a diamond pen before imaging using the fluorescence scanner.
A list of warnings read to Dr. Parazynski by Paolo A. Nespoli, the Italian astronaut who was choreographing the spacewalk from the station, included touching sharp edges of bolts, solar cells, hinges and other areas of the array.
Single spots or areas of the array with obvious blemishes were flagged and excluded from subsequent analyses.
Since the selection of which areas of the array to deprotect is done electronically, no mask is utilized and no alignment is necessary.
Spots or areas of the array with obvious blemishes were manually flagged and excluded from subsequent analysis.
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According to Lotien Huang, a researcher on the project, this could be improved by increasing the area of the array so that the molecules separate over a longer distance.
In non-GC mode, the individual arrays were confirmed to behave as microelectrodes arising from linear diffusion to the total area of the array.
The total active area of the array is therefore 32×16 mm2 while the total area of the detector (including 1 mm border dead area) is 34×18mm2.
Equation (4) is integrated over the CNT's surfaces to obtain the overall current, which is normalized by the current from a perfect array I PA. Figure 2 shows I PA and the overall current density, J PA, defined as the total current divided by the area of the array.
Initially the area of the array was 2.5 106 m2, which is to be compared with Agassiz's 105 m2, but in 1960, after Scarsi had returned to Milan, Linsley spread the detectors over an area of 107 m2.
Each calibration and negative controls from the Lucidea kit were spotted several times across the whole area of the array.
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