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The software divides the normalised peak areas of the patient by the average peak areas of reference samples (four reference samples from healthy individuals were introduced in each run).
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Access to hospital discharges outside the area of reference is therefore required.
Soil management treatments included conventional tillage (CT) and no-till (NT) and a selected adjacent area of reference vegetation (RV).
Distance to the nearest reference wetland explained perennial wetland richness in both regions, and area of reference wetlands within 15 km also was important in the WHP.
This dimension required a description of the technology and the pathology under assessment and, in addition, the definition of the real and potential catchment area of reference for the procedures.
Distortions due to this shift in the area of reference will be discussed with the results.
Intrasample normalization was by dividing the peak area of each probe's amplification product by the total area of reference probes only.
We consider the same area of reference (Czech Republic plus Slovakia) after the separation of the two countries in 1993 in order to be consistent with the earlier data.
For the case of Austria, it is however possible to keep the same (or a quite similar) area of reference throughout the entire time period.
The HPLC system was connected to a computer to quantify all compounds by comparing the area under the peaks with the area of reference standards with specific HPLC software (Chromatography Station for Windows).
The latter covers a geographical area of reference, with a population of up to 4,000 inhabitants and its work should essentially be performed in the community through home visits [ 13].
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