Platelet adhesion to collagen and thrombus formation in the flow chamber did not show significant differences in platelet covered area and average size of platelet covered areas of non-activated platelets of healthy dogs and dogs with infection (P > 0.05, unpaired t-test).
The application of TFMG to bare surfaces was shown to reduce the attachment area of human platelets by 77% and that of pig platelets by 63%.
The surface area of adherent platelets was measured using Volocity software (Improvision), while the number of adherent platelets was counted manually.
The platelet area (μm), the area of fibrin fibers (μm), the ratio of the minor and major axes of platelets, the ratio of the major and minor axes of platelets, and the number of α-granules found within platelets were measured for the cat clots.
The specific surface area of these platelets is 94.63 m2 g−1.
The parameters considered in cats were individual platelet area (μm), percentage of platelet area, fibrin fiber area (μm), percentage of fibrin fiber area, the ratio between the minor and major axes, the ratio between the major and minor axes of platelets, and the number of α-granules found within each platelet (n = 100 platelets for each activating substance).
The best area under the curve was given by ammonia concentrations, i, e., 0.78, when comparing areas of ammonia levels, platelet count and spleen longitudinal diameter at ultrasonography.
P-selectin is also very important in the recruitment and aggregation of platelets at areas of vascular injury and has also the ability to attach to the actin cytoskeleton through anchor proteins.
In contrast, in dogs with infection neither platelet covered area nor average size of platelet covered areas increased after platelet activation with PAR 4 agonist (P > 0.05, paired t-test).
In our flow chamber studies, platelet covered area and average size of platelet covered areas in dogs with infection and healthy dogs did not show significant differences at baseline.
