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After feeding for 24 h, areas of leaf discs consumed were measured.
The areas stained and areas of entire leaves, both in pixels, were used to calculate percentage areas of leaf stained [12] [13].
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The numbers of particles adsorbed per unit area of leaf differed significantly depending on the location at which the samples were collected.
Prior to drying the surface area of leaf blades was measured for each plant with a Licor LI-3100C (Licor, Nebraska, USA).
The area of leaf 8 from each plant was measured, and the cross-sectional area of palisade mesoplyll cells in leaf 8 was measured with the aid of a microscope.
A high SLA implies a thinner leaf and a lower density of nitrogen and chlorophyll per area of leaf.
Leaves from individual trees were harvested separately, and photographed to determine the area of leaf damage as described in [ 77].
After 24 h of feeding, we harvested the plants and the scanned their leaves to determine the total area of leaf tissue consumed.
An image analysis software (Adobe Photoshop v. 5.5, with image processing toolkit) was used to calculate the area of silicon as a proportion of the area of leaf material.
The leaf area (cm2 of leaf area shoot-1) and leaf area index (m2 of leaves per m2 of substrate) were calculated according to Giraud [ 30].
The accumulation of water in some areas of the leaf surface permitted those leaf areas to remain active.
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